Coding
Part:BBa_K2926052:Design
Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
Fusion protein of mCherry and M13 bacteriophage protein pVIII
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To enable proper protein folding of both subdomains we designed a coding sequence for a short glycine-serine linker in between them.
Source
mCherry was derived from the iGEM part BBa_J06504 and the phage protein pVIII from a helperphage plasmid purchased from NEB.